Journal: Virologica Sinica

Article Title: Function investigation of p11.5 in ASFV infection

doi: 10.1016/j.virs.2024.05.007

Figure Lengend Snippet: Silver staining was used to detect the enrichment of p72 interacting proteins. A Dynamic growth of ASFVGZ in IPAM and PK-15 ​cells in vitro . IPAM or PK-15 ​cells were infected (MOI ​= ​0.1) with ASFVGZ and viral genome copies were calculated at the indicated times post-infection. PK-15 ​cells ( B ) and IPAM cells ( C ) were infected with ASFVGZ at 0.1 MOI. Anti-p72 mAb were used for CO-IP 48 ​h after cell infection. p72-interacting proteins were eluted with protein G sepharose and analyzed on SDS-PAGE followed by silver staining. The normal mouse IgG as a negative control. The data were tested three times independently. Arrows indicate differential bands.

Article Snippet: The beads were washed 4 times with 1 mL phosphate-buffered saline (PBS) for 10 min, and boiled in a 5 × Protein SDS PAGE Loading Buffer (Novoprotein, Shanghai, China) for silver staining or Western blot analyses using One step PAGE Gel Fast Preparation Kit (MeilunBio, Dalian, China).

Techniques: Silver Staining, In Vitro, Infection, Co-Immunoprecipitation Assay, SDS Page, Negative Control